Tthssb

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August undefined, 2024

WebTthSSB appears to be positioned near the center of the (dT)60+n ssDNA overhangs though rapid diffusion. (A) A schematic illustration of our experimental design for (dT)60+n (n = 0, … WebJun 1, 2005 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand

Improvements of rolling circle amplification (RCA) efficiency and ...

WebOct 1, 2002 · TthSSB and TaqSSB monomer proteins contain 264 and 266 amino acids, respectively.The homology between these proteins is very high—82% identity and 90% … Webdate, offering an attractive alternative to TaqSSB and TthSSB in molecular biology applications, especially with using high temperature e. g. polymerase chain reaction (PCR). Background Single-stranded DNA-binding (SSB) proteins play an essential role in allin vivo processes involving ssDNA. They interact with ssDNA and RNA, in an independent earring necklace set

Enhancement of DNA, cDNA synthesis and fidelity at high …

WebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over linear targets. Crown", WebThe N-terminal domain is located in the region from amino acid 1 to 123 and the C-terminal domain is located between amino acids 124 and 264 or 266 in TthSSB and TaqSSB, … http://www.diva-portal.org/smash/record.jsf?pid=diva2:658627 ctb 14760

Novel thermostable ssDNA-binding proteins from Thermus ... - PubMed

WebThe presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus. TthSSB was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate Tth DNA polymerase during cDNA synthesis. National Category Biochemistry and Molecular Biology Identifiers WebMay 17, 2006 · We have overexpressed the native and mutant forms of TthSSB protein in Escherichia coli and purified them to homogeneity. In vitro, these proteins were found to … earring necklace sets weddingWebJun 1, 2005 · Here, we demonstrate the application of SSB-like protein from Thermus aquaticus (TaqSSB) in multiplex PCR of human Y-STR markers identification.The use of thermostable TaqSSB prevents or reduces primer dimmer formation, one of the particular problems known to cause inhibition of primer hybridization to the template and reduction … ct b-148

"Web(A and B) Representative single-molecule time traces (A) and average cross-correlation curves (B), for (dT)65+hp+3 alone and TthSSB-bound (dT)65+hp+3, obtained at 500 mM … " - Tthssb

Tthssb

http://www.100md.com/html/DirDu/2006/07/27/12/18/65.htm WebOct 1, 2002 · Europe PMC is an archive of life sciences journal literature.

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WebJun 21, 2006 · It is shown that DgeSSB is similar to Thermus/Deinococcus SSB in its biochemical properties and offers an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and for analytical purposes. To study the biochemical properties of single-stranded DNA-binding (SSB) protein from Deinococcus …

WebNov 15, 2003 · The thermostability of TthSSB was also studied. To this end, TthSSB was pre‐incubated at 94°C for different times, cooled down to 70°C, and immediately … WebExcellgen Tth-SSB [EG-171] - Return to List . Next

WebNov 1, 2003 · Europe PMC is an archive of life sciences journal literature. WebHere we show, using single-molecule fluorescence resonance energy transfer (FRET), that homodimeric bacterial SSB from Thermus thermophilus (Tth) is able to diffuse …

WebThermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to single-stranded DNA speciﬁcally over a temperature range of 4–80oC, and the wild-type protein could withstand incubation at 94oC for 2 min. Addition of TthSSB to PCR halved the elonga-tion time required for the DNA polymerases of

WebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not … earring necklace standWebA fixed quantity (20 pmol) of 5h-end fluorescein-labelled oligonucleotides (dT) 30 , (dT) 60 , (dT) 70 or (dT) 76 was incubated with 0, 5, 20, 50 and 200 pmol of TthSSB in 20 µl … ctb 162http://www.biotools.eu/documentospdf/DNA-Polymerase-030.pdf earring necklace ring braceletWebAug 8, 2005 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand earring nestWebAug 10, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the … earring of orfenWebNov 15, 2003 · The thermostability of TthSSB was also studied. To this end, TthSSB was pre‐incubated at 94°C for different times, cooled down to 70°C, and immediately subjected to bandshift assays. As shown in Figure 2 C, TthSSB could withstand up to 2 min incubation at 94°C, without any detectable loss of its ssDNA‐specific binding activity. earring of heren flyffWebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical … ctb152g1rw