Phospho-rps6

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August undefined, 2024

WebImmunohistochemical analysis of Phospho-RPS6 pSer240 in paraffin-embedded human breast carcinoma using Phospho-RPS6 pSer240 polyclonal antibody (Product # PA5-36759) at a dilution of 1:100. Western blot analysis of Phospho-S6 (Ser240) in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: HeLa treated with serum starved (24h) whole cell lysate (40 ... Web上海士锋生物为您提供rere抗体的参数及2024年最新报价，厂家专业的rere抗体售后服务团队，是您值得信赖的合作伙伴

Phospho-S6 Ribosomal protein (Ser236) Recombinant antibody

WebWB analysis using 80206-1-RR. Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80206-1-RR (Phospho-S6 Ribosomal protein (Ser236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. WebRPS6 Rabbit Polyclonal Antibody: 50μl: 593.00元: 加入购物车: AG3129: Phospho-S6 Ribosomal Protein (Ser235/236) Mouse Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3133: Phospho-S6 Ribosomal Protein (Ser235/Ser236) Rabbit Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3136: Phospho-S6 Ribosomal Protein (Ser240/244 ... bingley council planning

Phosphorylation of ribosomal protein S6 ... - Oxford …

WebCells were then intracellularly stained with 5 µg/mL (1:100 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. WebMar 9, 2024 · Levels of phosphorylated RPS6 (p-RPS6) and S6 kinases (p-S6K) are shown to indicate the effectiveness of rapamycin at inhibiting mTORC1 activity. WebDec 21, 2007 · The level of phosphorylated S6 ribosomal protein expression was predictive of early tumor response to the mTOR inhibitor, suggesting that this is a promising new predictive sarcoma marker for... bingley councillors

The tumor suppressor FLCN mediates an alternate mTOR pathway …

Recombinant Anti-RPS6 (phospho S235 + S236) antibody [SP50] …

WebMay 3, 2024 · (i) Ribosomal protein S6 (rpS6) is a downstream substrate of PI3K/akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double strand breaks. Phospho-rpS6 is reliably measurable by flow cytometry and thus has the potential to function as a biomarker of responsiveness to several therapeutic agents. WebNational Center for Biotechnology Information bingley college facebookWebWestern blot analysis of Phospho-RPS6 (Ser240, Ser244) in untreated (–) and treated (+) 293T whole cell extract using Phospho-RPS6 (Ser240, Ser244) polyclonal antibody ( Product # PA5-85822) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. bingley college where are they now

"WebAnimals were fasted overnight and refed ad lib for 4 h. Quantification of phospho-rpS6 is shown at the right, normalized to total rpS6. (*) P < 0.05 by Student's t-test. (D) Immunofluorescence staining of phospho-S6 (red) and TFE3 (green) in control (NTC) cells and cells lacking TSC1 or TSC2. Cells were starved or starved and restimulated with ... " - Phospho-rps6

Phospho-rps6

Web兔抗RPS6 (Phospho-Ser235)多克隆抗体由上海纪宁整理，包括兔抗RPS6 (Phospho-Ser235)多克隆抗体使用说明书、价格表、应用范围、使用注意事项及操作步骤等资讯。 WebDec 21, 2024 · Ribosomal protein S6 (RPS6) is a component of the 40S small ribosomal subunit and participates in the control of mRNA translation. Additionally, phospho (p) …

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WebPhospho-S6 Ribosomal Protein (Ser240/244) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 240 and 244. This antibody does not detect S6 ribosomal protein phosphorylated … WebCells were then intracellularly stained with 2 µg/mL of purified anti-RPS6 Phospho (Ser244) antibody (clone A18024A) overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.

WebApr 1, 2024 · Phospho-rpS6 levels were significantly attenuated (3.9 ± 1.9 pre-exercise vs. 1.4 +/0.4 post-exercise, p = 0.04) (Fig. 1). Attenuation of Phospho-rpS6 levels was strongly positively correlated with loss of intrahepatic fat ( r = 0.98); no correlation was observed between attenuation of Phospho-rpS6 and change in VO2max ( r = 0.20) or change in ... WebRibosomal protein S6 (rpS6 or eS6) is a component of the 40S ribosomal subunit and is therefore involved in translation. Mouse model studies have shown that phosphorylation …

WebDot blot analysis of RPS6 (phospho S240 + S244) labeled with ab215214 at 1/1000 dilution. Lane 1: RPS6 (phospho S240 + S244) peptide. Lane 2: RPS6 (phospho S240) peptide. Lane 3: RPS6 (phospho S244) peptide. Lane 4: RPS6 non-phospho peptide. Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody. WebThe phosphorylation of ribosomal protein S6 (rpS6) has been described for the first time about four decades ago. Since then, numerous studies have shown that this modification …

WebMar 9, 2024 · Flow Cytometry - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691) Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial …

WebFeb 6, 2024 · We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully... d1s wifiWebAn immobilized capture antibody specific for Ribosomal Protein S6 / RPS6 binds both phosphorylated and unphosphorylated Ribosomal Protein S6 / RPS6. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format. Complete Your Research bingley court canterbury managementWebQuality Control: the Phospho-RPS6 (S240/S244) assay kit is routinely tested against Insulin-treated MCF7 lysates. MCF7 cells were cultured in a T175 flask to 90% confluence and stimulated with 10 µM of insulin for 60 min at RT. Following cell lysis using 16 mL of 1X Lysis Buffer 4, lysates bingley community centreWebProduct name Anti-RPS6 (phospho S235) antibody [EP1338 (2)Y] - Ribosome Marker See all RPS6 primary antibodies Description Rabbit monoclonal [EP1338 (2)Y] to RPS6 (phospho … bingley council bingley court canterbury council tax bandWebAll lanes : Anti-RPS6 (phospho S240 + S244) antibody [EPR20770] (ab215214) at 1/5000 dilution Lane 1 : MCF7 (human breast adenocarcinoma cell line) grown in serum-free media overnight, whole cell lysate Lane 2 : MCF7 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes, whole cell lysate Lane 3 : NIH/3T3 (mouse embryonic … d1 swimming times women\\u0027sWebMar 11, 2011 · Carboxyl-terminal phosphorylation of rpS6 is regulated by at least two signal transduction pathways. The p70 ribosomal S6 kinases, S6K1 and S6K2, play a major role in rpS6 C terminus phosphorylation in response to insulin, serum, and amino acid stimulation ( … d1 swim conferences