WebAnti-phospho-AMPK (Thr172), clone 5J23 ZooMAb, Cat. No. ZRB1213, is a specific recombinant rabbit monoclonal antibody that detects AMPK alpha phosphorylated on threonine 172 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Peptide Inhibition Assay, and Western Blotting. Web(A) Representative western blot of expression levels of Phospho-AMPK, Phospho-AKT, AKT, Phospho-ERK1/2, ERK1/2, N-Cadherin and E-Cadherin in MTT wt, MTT SDHB sil and fibroblasts treated or not with 4 mM of metformin for 72 h. α-tubulin immunoblot was used as loading control.
Phospho-AMPKα (Thr172) (40H9) Rabbit mAb - Cell …
WebApr 8, 2024 · Membranes were incubated overnight with primary antibodies against phospho-AMPK (Thr172) (Cell Signaling), phospho-ACC (Ser79 ... then either phospho AMPK antibody (Cell Signaling, 2535) at 25 ng/well or total AMPK antibody (Cell Signaling, 5831) at 100 ng/well, diluted in 1% MSD Blocker A, were added to respective wells and … WebPhospho (Thr172, Thr172) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr172 of human AMPKα protein. Validations Biological Strategies … dynaheat hot water systems
Phospho-AMPK alpha-1 (Thr172) Polyclonal Antibody
WebImmunocytochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in C2C12 cells. Samples were treated with PFA, permeabilized in 0.1% saponin prior to blocking in 10% serum (45 min at 37°C), and incubated with polyclonal antibody (Product # PA5-114550) at a dilution of 1:400 (1 hr at 37°C). WebPhospho-AMPKα (Thr172) (40H9) Rabbit mAb detects endogenous AMPKα1 only when phosphorylated at threonine 183 and endogenous … WebAnti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody Description Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Host … dynaheat heat pump review