Ionomycin thermo
Web2 feb. 2024 · Cells were then stimulated for 14 hours with 3 µg/ml CpG 7909 (PF-3512676 provided by Pfizer and Coley Pharmaceutical Group, Wellesley MA), after which 50 ng/ml PMA (Sigma-Aldrich) and 500 ng/ml Ionomycin (Thermo Fischer) were added. Web29 mrt. 2024 · After 12 h of incubation, protein transport inhibitor (Thermo Scientific, Netherlands) was added to stop the transport of proteins out of the Golgi apparatus. Cell stimulation cocktail containing PMA-ionomycin (Thermo Scientific, The Netherlands) was used as a positive control stimulant.
Ionomycin thermo
Did you know?
Web27 aug. 2013 · The PMA/ionomycin combination significantly increased (p < 0.01 or p < 0.05) the production of IL-2, IFN-γ, TNF-α, TGF-β and RANTES within 4 to 6 h. In contrast, Con A had lesser effects on cytokine production as it only increased significantly (p < 0.05) the level of RANTES. WebFor a positive control, add Ionomycin (a calcium ionophore, Cat. no. I24222) to a final concentration of 10 μM for a large increase in cytosolic calcium concentration. 30 October 2013 Purchaser Notification Corporate Headquarters 5791 …
WebMeer informatie over Ionomycin. Samen zetten we ons in voor de wetenschap door ons ruime productaanbod, onze diensten, excellente processen en onze deskundige medewerkers Web9 jan. 2024 · Cells were grown until confluency (~63 × 10 5 cell count) and then stimulated to release 5-HT using allyl isothiocyanate (AITC) and ionomycin (Thermo Fisher Scientific). AITC was purchased as an oil-based solution, which was first diluted in DMSO, then diluted further in diH 2 O to result in a 3 mM AITC stock solution in 30% DMSO.
WebIntracellular cytokine and chemokine detection often requires stimulation of defined cell types prior to analysis. Refer to this list below to learn how to induce commonly analyzed … Web22 jul. 2024 · Ionomycin is one of the most widely used AOA chemical reagents in the clinic (Vanden Meerschaut et al., 2014). Many studies ... (#35502, #35557 or #35512, Thermo, USA) at 4°C overnight, and the nuclei were stained with DAPI. After staining, the samples were mounted on coverslips and imaged with a Nikon A1 confocal microscope ...
WebNext, after the permeabilization at 4 °C for 20 min, 1 × 10 6 cells were incubated with 0.25 μg PerCP/Cy5.5-conjugated anti-mouse CXCR3 (Thermo fisher, Scientific, Pittsburgh, PA, USA), 1 μg APC-conjugated anti-mouse CCR4 (Biolegend), 0.13 μg FITC conjugated anti-mouse CD25 (Proteintech), 0.125 μg PE-conjugated anti-mouse Foxp3 (after …
Web15 apr. 2024 · For cytokine staining, the same sets of cells were seeded in 48-well plates, followed by incubation with T-cell media containing 10 nmol/L of phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich), 500 nmol/L of ionomycin (Thermo Fisher Scientific), and 1 μg/mL of brefeldin (BioLegend) for 4 hours at 37°C. northernapex.comWebThermo Scientific Chemicals Ionomycin, 96% 1 mg, Each Quantity 1 mg 5 mg Specification Sheet Certificates SDS Catalog number: J62448.MCR also known as … northern apostle radioWebThe Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. Treatment with … northern anvil strainWebIonomycin is an effective Ca 2+ ionopohre that is commonly used to both modify intracellular Ca 2+ concentrations and to calibrate fluorescent Ca 2+ indicators when studying the regulatory properties of Ca 2+ in cellular processes. Ionomycin also … Ionomycin is an effective Ca 2+ ionopohre that is commonly used to both modify … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes how to rewire a light switchWeb20 mei 2024 · In this protocol, we describe robust and optimized ex vivo polyclonal activation and cell culture conditions to measure drug treatments' effects on primary human CD8+ T cell activation and cytolytic potential. We provide streamlined methods for measuring effector cytokines and activation markers of CD8+ T cells via flow cytometry. northern apc liverWeb9 jan. 2024 · Ionomycin Calibration. Ionomycin (Thermo Fisher Scientific, Waltham, MA, USA; Abcam) was used to calibrate derived calcium concentrations from ratios of emitted fluorescence given by 340/380 nm UV excitation determined with Fura-2AM and Fura-4F. northern apkWeb21 jan. 2024 · To tease out whether the discrepancy is specific for IFNγ, we next tested another widely studied inflammatory cytokine, IL-17, which is mainly produced by γδ-T cells in various types of inflammatory diseases (6, 7).Using the same 4T1 model, a remarkable reduction in the percentages of IL-17 + γδ-T cells was found based on ICS analysis … northern apartments oulu