Incipient cytorrhysis assay

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August undefined, 2024

WebApr 7, 2024 · Appressorium turgor pressure was analyzed by the incipient cytorrhysis assay using 1–4 M of glycerol solution, as described by Wang et al. (2024). The experiment was conducted three times with at least 100 structures per replicate. Onion penetration assays were conducted as described by Wang et al. (2024). WebApr 11, 2014 · We applied incipient cytorrhysis to investigate the appressorial turgor pressure of P. pachyrhizi. Mature appressoria were …

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WebJan 26, 2016 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5 M glycerol solution as previously described (Foster et al. 2003). The onion penetration assay was performed as previously described (Wang et al. 2013). Onion epidermal strips were isolated and 40 μl of conidial suspension was inoculated onto the adaxial surface. WebFeb 9, 2012 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1–5 m glycerol solution (Howard et al., 1991). Droplets (20 µL) of the conidial suspension (5 × 10 4 spores/mL) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature. green leather chesterfield loveseat

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WebNational Center for Biotechnology Information WebAug 4, 2014 · (A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying … WebJan 14, 2014 · The incipient-cytorrhysis assay was performed to measure appressorial turgor. Conidia in 20 μl droplets (2×10 5 /ml) were allowed to form appressoria on plastic coverslips for 48 h. Water surrounding the conidia was removed carefully and replaced with 20 μl 0.5–4.0 M glycerol. The number of collapsed appressoria was counted after 10 min. green leather chesterfield couch

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WebAntibody Internalization. Antibodies specific for cell surface receptors induce receptor-mediated endocytosis when they bind. They are commonly studied as vehicles for … WebDec 27, 2016 · (C) Incipient cytorrhysis assay to measure appressorium turgor generation in Guy11, Δcds1, and Δchk1Δcds1 mutants in the presence or absence of HU exposure … green leather chairsWebJul 5, 2016 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1–4 m glycerol solution (Howard et al., 1991). Droplets (20 µL) of the conidial suspensions (5 × 10 4 spores/mL) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature. green leather chair slipcover

"WebJul 28, 2015 · The appressoria formed at 24 h and 48 h post incubation were used in the incipient-cytorrhysis assay to measure the appressorial turgor as described (34). The experiments were replicated three times with more than 200 appressoria were counted for each strain. ... The incipient-cytorrhysis method was used to compare the appressorial … " - Incipient cytorrhysis assay

Incipient cytorrhysis assay

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WebDec 27, 2016 · (C) Incipient cytorrhysis assay to measure appressorium turgor generation in Guy11, Δcds1, and Δchk1Δcds1 mutants in the presence or absence of HU exposure … Web(A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying concentrations (1–4 M). …

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WebNov 16, 2024 · An incipient cytorrhysis assay was carried out by allowing appressoria to form in water on borosilicate cover slips for 24 hr, after which the water was replaced with a range of aqueous glycerol ranging from 0.25 to 2.5M and, after 30 min, the frequency of cytorrhysis determined ( de Jong et al. 1997 ). WebAug 3, 2024 · Glycogen staining and cytorrhysis assays Appressorium turgor was measured using an incipient cytorrhysis assay. Twenty microlitres of the conidial suspension (5 × 10 4 spores/ml) was placed on hydrophobic coverslips and incubated in a humid chamber for 24 h at room temperature.

WebIncipient cytorrhysis determined the turgor pressure in appressoria of P. pachyrhizi to be equivalent to 5.13 MPa. MZM data revealed that osmotically active sugar alcohols only … WebSep 27, 2024 · Incipient cytorrhysis assay Appressorial turgor was estimated using incipient cytorrhysis assays ( Howard et al., 1991 ). Droplets (20 µL of 1 × 10 5 conidia/mL) of M. …

WebJul 1, 2024 · Appressorium turgor was measured following the incipient cytorrhysis (cell collapse) assays ( Howard et al., 1991 ). The plastic coverslips were prepared to incubate droplets (20 μl) of conidial suspension (1 × 10 5 conidia/mL). The setup was placed in a humid chamber for 24 h. WebApr 1, 2002 · CYP1 putatively encodes a mitochondrial and cytosolic form of cyclophilin, and targeted gene replacement has shown that CYP1 acts as a virulence determinant in rice blast. Cyp1 mutants show reduced virulence and are impaired in associated functions, such as penetration peg formation and appressorium turgor generation.

WebAug 20, 2024 · To elucidate the defect in appressorium penetration caused by myriocin/FB1, we measured the internal turgor pressure within appressoria using an …

WebDec 20, 2024 · While a confocal microscope was used for investigation of subcellular localizations, light microscopy using Olympus DP80 (Tokyo, Japan) was utilized for conidiophores formation assay, cytorryhsis incipient assay, invasive hyphae development and appressorium penetration and glycogen degradation. 2.5. fly high burnout syndromes lyricsWebIn this study, we used incipient cytorrhysis to determine the turgor pressure of the appressoria of P.pachyrhizi. MZM data unravelled the contribution of osmolytically active … green leather checkbook coverWeb1.4.2 Retesting a current lot due to problems in the assay. Reagent Titration for the ICS Assay . FH-HVTN-P0015 Page 4 of 11 Version 3.0 1.4.3 Sometimes antibodies may … green leather chesterfield 2 seater sofaWebincipient cytorrhysis assays indicate that turgor pressure begins to be generated in the appressorium from 16 h (Fig. 1) and penetration of the rice cuticle can occur from 24 h … green leather chairs in living roomWebApr 16, 2024 · To determine whether the appressorial turgor pressure was altered in ∆mopex13 and ∆mopex14, we performed an incipient cytorrhysis assay ). The collapse rates of the appressoria of ∆mopex14 treated with glycerol were increased compared with the wild type, suggesting a reduction of the turgor generation in ∆mopex14 appressoria. green leather chair tuftedWebFeb 18, 2024 · Considering that the germinating and appressorial formation were normal in the Mohat1 mutant (Table S1), we measured the internal concentration of solute and the turgor pressure within appressoria by the incipient cytorrhysis (cell collapse) assay using 1–4 molar of glycerol [ 45 ]. green leather chair blue velvet sofaWeb(A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying concentrations (1-4 M). fly high burnout syndromes anime