WebThe GenScript Glutathione MagBeads are superparamagnetic beads, average 40 µm in diameter, coupled with reduced glutathione (GSH). The beads are supplied as 25% … WebPull-Down Glutathione Agarose beads 90 µm Protein G Resin. AC, Purif Protein G Agarose beads ... Resuspend the PureCube Ni-NTA MagBeads by vortexing. Transfer 40 μL of the 25% magnetic beads suspension onto the lysate. Note: Depending on the protein expression rate and desired protein concentration, the elution volume can be adjusted …

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WebThe GenScript Ni-Charged MagBeads are average 40 μm in size, superparamagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline ... WebApr 6, 2024 · Then, 1 ml of GST and GST-CcSSP1 sonicated soluble supernatant was incubated with 30 μl PureCube Glutathione MagBeads (Cube Biotech) at 4°C for 1 h. The supernatant was then removed, and MagBeads were added to the supernatant expressing His-MaPR1 and incubated at 4°C for 1 h. GST fusion proteins were eluted with reduced … jefferson high school lafayette indiana

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WebMar 28, 2024 · One mL of GST-tagged Avrblb2 fusion protein extracts were added Glutathione MagBeads and incubated at 4°C for 2 h with gentle rotation. Beads were centrifuged at 200 × g for 2 min and washed five times with 1 ml TBST. 1 ml of HIS-CaM extracts in TBST containing either 2 mM CaCl 2 or 5 mM EGTA were added to the GST … WebThe GenScript Glutathione MagBeads are developed for quick and efficient small‐scale purification of recombinant glutathione‐S‐transferase (GST)-tagged fusion proteins from … oxo organic hair straightening near me