Fastpfu buffer
WebEach batch of TransStart FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. Storage Buffer. 50 … WebEach batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. Restrictions For …
Fastpfu buffer
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WebMar 8, 2024 · We then collected below-ground root biomass to a depth of 30 cm using soil cores (diameter 7 cm) in each of these five quadrats as well. Roots were collected by … Web本发明公开一种基于环境DNA技术监测淡水底栖动物群落多样性的方法，包括步骤：(1)采集表层水样；(2)水样过滤与eDNA提取；(3)PCR扩增；(4)高通量测序，获得OTU的代表序列；(5)底栖动物比对数据库的建立；(6)比对建立的底栖动物数据库，对待测样品OTUs代表性序列进行物种注释。本发明取样简单、人力 ...
WebMar 18, 2024 · Cell lysis buffer Store it at room temperature (22°C–26°C, hereafter) for up to 6 months. CRITICAL: SDS is toxic. Wear gloves and a mask to avoid skin exposure and inhalation. TE buffer Store it at room temperature for up to 1 year. 1× B&W buffer Store it at room temperature for up to 1 year. 10 mM Tris-HCl, pH7.5 WebMar 11, 2024 · A 20-μL PCR reaction system contained 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL each of the 338F and 806R primers, 0.4 μL of FastPfu polymerase, 10 ng of template DNA, 0.2 μL of BSA, and residual ddH 2 O.
WebThe amplicons were purified from 2% (w/v) agarose gels using an AxyPrep DNA gel extraction kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer's protocol. The SMRTbell libraries were prepared from the amplified DNA using blunt-ligation according to the manufacturer's protocol (Pacific Biosciences, Menlo Park, CA, USA). WebFastPfu FLY Sets Higher Standards of High-Fidelity DNA Polymerase Performance. Whereas Phusion and Q5 failed, FastPfu FLY succeeded easily at... 1-877-661-4711 …
Web引物ITS3F（5'-GCATCGATGAAGAACGCAGC-3'）和ITS4R（5'-TCCTCCGCTTATTGATATGC-3'）：武汉天一辉远生物科技有限公司；QIAGEN DNeasy mericon Food Kit试剂盒：德国QIAGEN公司；5×FastPfu Buffer、2.5 mmol/L脱氧核糖核苷三磷酸（deoxy-ribonucleoside triphosphates，dNTPs）、FastPfu Polymerase、牛血 …
Webmixtures containing 4 lL of 5X FastPfu Buffer, 2 lL of 2.5 mM dNTPs, 0.8 lL of each primer (5 lM), 0.4 lL of FastPfu Polymerase, and 10 ng of template DNA. The PCR products were extracted from a 2% agarose gel and further purified using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA). The products were quantified using chad raicheWebJan 4, 2024 · The extracted DNA was used as a template for 16S rRNA gene PCR amplification, and barcoded primers were used for PCR. The following amplification system was used: 4 μL TransStart™ 5 × FastPfu buffer, 2 μL 2.5 mM dNTPs mix, 0.5 μL 5 μM 338F and 806R, 0.4 μL TransStart™ FastPfu polymerase, 10 ng DNA template, and … hansen tree tableclothWebDec 1, 2024 · The mobile phase consisted of acetonitrile as Solvent A and 1% formic acid as Solvent B. The gradient elution program of the solution include: 55% A at 0–20 min; … hansen truck repair